LOCUS hSERT pcDNA3 8025 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS hSERT pcDNA3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8025)
AUTHORS Ramamoorthy S, Bauman AL, Moore KR, Han H, Yang-Feng T, Chang AS,
Ganapathy V, Blakely RD
TITLE Antidepressant- and cocaine-sensitive human serotonin transporter:
molecular cloning, expression, and chromosomal localization.
JOURNAL Proc Natl Acad Sci U S A. 1993 Mar 15. 90(6):2542-6.
PUBMED 7681602
REFERENCE 2 (bases 1 to 8025)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8025
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 972..988
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 972..988
/label=pBluescriptSK
/note="For pBluescript vector"
primer_bind complement(3538..3554)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3539..3555)
/label=pBluescriptKS
/note="For pBluescript vector"
promoter complement(3578..3596)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(3579..3596)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(3616..3633)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 3622..3846
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3892..4320
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3979..3998)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4189..4210
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4329..4349)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4334..4663
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4514..4649
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4576..4595
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4730..5524
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4784..4803)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5394..5413
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 5698..5819
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5735..5754)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5789..5808
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5868..5884)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5868..5884)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5881..5903)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5892..5908
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5916..5946)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5961..5982
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6099..6116)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6270..6858)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6350..6369)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7029..7889)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7652..7671
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7890..7994)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat atccatcaca
961 ctggcggccg ctctagaact agtggatccc ccgggctgca ggaattccca aatccaagca
1021 cccagagatc aattgggatc cttggcagat ggacatcagt gtcatttact aaccagcagg
1081 atggagacga cgcccttgaa ttctcagaag cagctatcag cgtgtgaaga tggagaagat
1141 tgtcaggaaa acggagttct acagaaggtt gttcccaccc caggggacaa agtggagtcc
1201 gggcaaatat ccaatgggta ctcagcagtt ccaagtcctg gtgcgggaga tgacacacgg
1261 cactctatcc cagcgaccac caccacccta gtggctgagc ttcatcaagg ggaacgggag
1321 acctggggca agaaggtgga tttccttctc tcagtgattg gctatgctgt ggacctgggc
1381 aatgtctggc gcttccccta catatgttac cagaatggag ggggggcatt cctcctcccc
1441 tacaccatca tggccatttt tgggggaatc ccgctctttt acatggagct cgcactggga
1501 cagtaccacc gaaatggatg catttcaata tggaggaaaa tctgcccgat tttcaaaggg
1561 attggttatg ccatctgcat cattgccttt tacattgctt cctactacaa caccatcatg
1621 gcctgggcgc tatactacct catctcctcc ttcacggacc agctgccctg gaccagctgc
1681 aagaactcct ggaacactgg caactgcacc aattacttct ccgaggacaa catcacctgg
1741 accctccatt ccacgtcccc tgctgaagaa ttttacacgc gccacgtcct gcagatccac
1801 cggtctaagg ggctccagga cctggggggc atcagctggc agctggccct ctgcatcatg
1861 ctgatcttca ctgttatcta cttcagcatc tggaaaggcg tcaagacctc tggcaaggtg
1921 gtgtgggtga cagccacctt cccttatatc atcctttctg tcctgctggt gaggggtgcc
1981 accctccctg gagcctggag gggtgttctc ttctacttga aacccaattg gcagaaactc
2041 ctggagacag gggtgtggat agatgcagcc gctcagatct tcttctctct tggtccgggc
2101 tttggggtcc tgctggcttt tgctagctac aacaagttca acaacaactg ctaccaagat
2161 gccctggtga ccagcgtggt gaactgcatg acgagcttcg tttcgggatt tgtcatcttc
2221 acagtgctcg gttacatggc tgagatgagg aatgaagatg tgtctgaggt ggccaaagac
2281 gcaggtccca gcctcctctt catcacgtat gcagaagcga tagccaacat gccagcgtcc
2341 actttctttg ccatcatctt ctttctgatg ttaatcacgc tgggcttgga cagcacgttt
2401 gcaggcttgg agggggtgat cacggctgtg ctggatgagt tcccacacgt ctgggccaag
2461 cgccgggagc ggttcgtgct cgccgtggtc atcacctgct tctttggatc cctggtcacc
2521 ctgacttttg gaggggccta cgtggtgaag ctgctggagg agtatgccac ggggcccgca
2581 gtgctcactg tcgcgctgat cgaagcagtc gctgtgtctt ggttctatgg catcactcag
2641 ttctgcaggg acgtgaagga aatgctcggc ttcagcccgg ggtggttctg gaggatctgc
2701 tgggtggcca tcagccctct gtttctcctg ttcatcattt gcagttttct gatgagcccg
2761 ccacaactac gacttttcca atataattat ccttactgga gtatcatctt gggttactgc
2821 ataggaacct catctttcat ttgcatcccc acatatatag cttatcggtt gatcatcact
2881 ccagggacat ttaaagagcg tattattaaa agtattaccc cagaaacacc aacagaaatt
2941 ccttgtgggg acatccgctt gaatgctgtg taacacactc accgagagga aaaaggcttc
3001 tccacaacct cctcctccag ttctgatgag gcacgcctgc cttctcccct ccaagtgaat
3061 gagtttccag ctaagcctga tgatggaagg gccttctcca cagggacaca gtctggtgcc
3121 cagactcaag gcctccagcc acttatttcc atggattccc ctggacatat tcccatggta
3181 gactgtgaca cagctgagct ggcctatttt ggacgtgtga ggatgtggat ggaggtgatg
3241 aaaaccaccc tatcatcagt taggattagg tttagaatca agtctgtgaa agtctcctgt
3301 atcatttctt ggtatgatca ttggtatctg atatctgttt gcttctaaag gtttcactgt
3361 tcatgaatac gtaaactgcg taggagagaa cagggatgct atctcgctag ccatatattt
3421 tctgagtagc atatagaatt ttattgctgg aatctactag aaccttctaa tccatgtgct
3481 gctgtggcat caggaaagga agatgtaaga agctaaggaa ttcgatatca agcttatcga
3541 taccgtcgac ctcgagcatg catctagagg gccctattct atagtgtcac ctaaatgcta
3601 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
3661 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
3721 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
3781 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
3841 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
3901 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
3961 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
4021 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
4081 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
4141 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
4201 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
4261 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
4321 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
4381 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
4441 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
4501 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
4561 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
4621 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
4681 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
4741 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
4801 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
4861 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
4921 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
4981 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
5041 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
5101 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
5161 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
5221 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
5281 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
5341 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
5401 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
5461 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
5521 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
5581 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
5641 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
5701 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
5761 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
5821 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
5881 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
5941 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
6001 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
6061 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
6121 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
6181 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
6241 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
6301 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
6361 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
6421 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
6481 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
6541 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
6601 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
6661 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
6721 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
6781 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
6841 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
6901 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
6961 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
7021 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
7081 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
7141 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
7201 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
7261 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
7321 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
7381 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
7441 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
7501 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
7561 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
7621 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
7681 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
7741 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
7801 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
7861 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
7921 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
7981 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
